Integrated Physiological, Transcriptomic, and Metabolomic Analyses Revealed Molecular Mechanism for Salt Resistance in Soybean Roots

International Journal of Molecular Sciences

Guohong Zhang¹  Xiaomin Wang²  Xiaofan Na²  Yurong Bi²  Keke Li²  Juan Qin²  Jie Jin²  Shengwang Wang²  Jianfeng Wang³ 

[1] Institute of Dryland Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China;State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;

关键词: transcriptome;    metabolome;    amino acid;    nitrogen metabolism;    nitrogen use efficiency;    TCA cycle;   

DOI  :  10.3390/ijms222312848

来源: DOAJ

【 摘 要 】

Salinity stress is a threat to yield in many crops, including soybean (Glycine max L.). In this study, three soybean cultivars (JD19, LH3, and LD2) with different salt resistance were used to analyze salt tolerance mechanisms using physiology, transcriptomic, metabolomic, and bioinformatic methods. Physiological studies showed that salt-tolerant cultivars JD19 and LH3 had less root growth inhibition, higher antioxidant enzyme activities, lower ROS accumulation, and lower Na⁺ and Cl^- contents than salt-susceptible cultivar LD2 under 100 mM NaCl treatment. Comparative transcriptome analysis showed that compared with LD2, salt stress increased the expression of antioxidant metabolism, stress response metabolism, glycine, serine and threonine metabolism, auxin response protein, transcription, and translation-related genes in JD19 and LH3. The comparison of metabolite profiles indicated that amino acid metabolism and the TCA cycle were important metabolic pathways of soybean in response to salt stress. In the further validation analysis of the above two pathways, it was found that compared with LD2, JD19, and LH3 had higher nitrogen absorption and assimilation rate, more amino acid accumulation, and faster TCA cycle activity under salt stress, which helped them better adapt to salt stress. Taken together, this study provides valuable information for better understanding the molecular mechanism underlying salt tolerance of soybean and also proposes new ideas and methods for cultivating stress-tolerant soybean.

【 授权许可】

Unknown