Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen

International Journal of Molecular Sciences

Sungmin Hwang¹  Rylee K. Hackley¹  Amy K. Schmid¹  Nikita E. Chavarria²  Julie A. Maupin-Furlow² 

[1] Department of Biology, Duke University, Durham, NC 27708, USA;Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA;

关键词: halophilic archaea;    nitrogen metabolism;    ammonium uptake;    transcriptome;    alanine transport;    transcription factor;    lrp-type;   

DOI  :  10.3390/ijms20194784

来源: DOAJ

【 摘 要 】

Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.

【 授权许可】

Unknown