Vibrio metschnikovii, a Potential Pathogen in Freshwater-Cultured Hybrid Sturgeon

Animals

Xudong Li¹  Mengwei Zhang²  Lingbing Zeng²  Mingyang Xue²  Xihua Chen²  Zidong Xiao²  Wei Liu²  Yuding Fan²  Zhipeng Chu²  Yong Zhou²  Feilong Gong³ 

[1] Henan Fishery Technical Extension Station, Zhengzhou 450008, China;Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;Zhengzhou Fishery Technical Extension Station, Zhengzhou 450006, China;

关键词: aquaculture;    biochemical identification;    histopathology;    hematological parameters;    drug sensitivity;   

DOI  :  10.3390/ani12091101

来源: DOAJ

【 摘 要 】

In July 2021, a disease with a high mortality rate broke out in freshwater cultured hybrid sturgeon in Zhengzhou, Henan Province. A dominant strain, H-701, was isolated from diseased fish; physiological changes in diseased fish were investigated and molecular identification, biochemical characterization, and pathogenicity and drug sensitivity tests of H-701 were performed. The 16S rRNA gene sequence of H-701 was 99.86% homologous with that of Vibrio metschnikovii in GenBank. The 50% lethal dose of H-701 was 3.72 ± 0.929 × 10⁴ CFU/g fish weight. The proportion of monocytes, neutrophils, and eosinophils in the blood of diseased sturgeon increased significantly, whereas the proportion of lymphocytes decreased. In diseased fish, the serum levels of total protein, albumin, globulin, and alkaline phosphatase decreased significantly, and those of aspartate aminotransferase, alanine aminotransferase, and complement C3 increased significantly. There were obvious pathological changes in several tissues of the diseased fish. H-701 was sensitive to antibiotics such as florfenicol, enrofloxacin, and doxycycline. This study not only demonstrated that V. metschnikovii was the cause of death of a large number of hybrid sturgeon but also revealed its potential risk in hybrid sturgeon aquaculture. The results provide a basis for the diagnosis and prevention of this disease.

【 授权许可】

Unknown