Authentication of the Bilberry Extracts by an HPLC Fingerprint Method Combining Reference Standard Extracts

Molecules

Bingbing Liu¹  Weidong Yan¹  Tiantian Hu¹ 

[1] Department of Chemistry, Zhejiang University, Hangzhou 310027, China;

关键词: bilberry extract;    anthocyanins;    HPLC;    fingerprint;    authentication;   

DOI  :  10.3390/molecules25112514

来源: DOAJ

【 摘 要 】

A simple and fast high-performance liquid chromatography (HPLC) fingerprint method combining reference standard extract for the identification of bilberry extract was developed and validated. Six batches of bilberry extract collected from different manufactures were used to establish the HPLC fingerprint. Other berry extracts—such as blueberry extracts, mulberry extracts, cranberry extracts, and black rice extracts—were also analyzed for their HPLC chromatograms. The fingerprints of five batches of bilberry extract showed high similarities, while one batch was distinguished from others. Additionally, the content of anthocyanin Cyanidin-3-O-glucoside (Cy-3-glc) in each berry extract was analyzed and compared. The results indicate that this HPLC fingerprint method, combining reference standard extracts, could be used for the authentication and quality control of bilberry extracts.

【 授权许可】

Unknown