Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion

Journal of Clinical Medicine

Marjan Motiei¹  David Rumpik²  Tatana Rumpikova²  Andrea Cela³  Zdenek Glatz³  Katerina Tvrdonova⁴  Reza Khalili⁵  Katerina Vaculikova⁶  Tomas Saha⁶ 

[1] Centre of Polymer Systems, Tomas Bata University in Zlin, Třída Tomáše Bati 5678, 76001 Zlin, Czech Republic;Clinic of Reproductive Medicine and Gynaecology, 76001 Zlin, Czech Republic;Department of Biochemistry, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic;Department of Biology, Faculty of Sciences, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic;Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 12808 Prague, Czech Republic;Footwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou 3685, 76001 Zlin, Czech Republic;

关键词: metabolomics;    capillary electrophoresis;    embryo development;    culture media;   

DOI  :  10.3390/jcm9124094

来源: DOAJ

【 摘 要 】

The selection of a highly-viable single embryo in assisted reproductive technology requires an acceptable predictive method in order to reduce the multiple pregnancy rate and increase the success rate. In this study, the metabolomic profiling of growing and impaired embryos was assessed on the fifth day of fertilization using capillary electrophoresis in order to find a relationship between the profiling and embryo development, and then to provide a mechanistic insight into the appearance/depletion of the metabolites. This unique qualitative technique exhibited the appearance of most non-essential amino acids and lactate, and depleting the serine, alanyl-glutamine and pyruvate in such a manner that the embryos impaired in their development secreted a considerably higher level of lactate and consumed a significantly higher amount of alanyl-glutamine. The different significant ratios of metabolomic depletion/appearance between the embryos confirm their potential for the improvement of the prospective selection of the developed single embryos, and also suggest the fact that pyruvate and alanyl-glutamine are the most critical ATP suppliers on the fifth day of blastocyst development.

【 授权许可】

Unknown