Characterization of Complex Protein Mixtures from Human Plasma Fractionation by Capillary Electrophoresis and Mass Spectrometry

proteins;capillary electrophoresis

Frantz, Neha Joshi ; Morteza G. Khaledi, Committee Chair,Frantz, Neha Joshi ; Morteza G. Khaledi ; Committee Chair

University:North Carolina State University

关键词: proteins;    capillary electrophoresis;   

Others  :  https://repository.lib.ncsu.edu/bitstream/handle/1840.16/436/etd.pdf?sequence=1&isAllowed=y

美国|英语

来源: null

【 摘 要 】

The purpose of the research has been to develop a simple, quantitative method for characterizing complex protein mixtures using Cohn Fractionation IV-1 Paste as an example.This process step is the starting material for the plasma fractionation process which leads to purified alpha1-antitrypsin, and is an example of a complex protein matrix.Currently, CE analysis of complex plasma samples involves a Borate-NaCl buffer system which does not provide adequate resolution to quantitate protein composition.The goal of this research is to discover optimum CE separation conditions, which would allow rapid, efficient, and inexpensive characterization of these complex samples.A simple, reproducible system utilizing SDS has been developed which provides better resolution and quantitation of complex protein mixtures.This new method is referred to as 'CE-SDS,' and although it is an improvement over the current method, orthogonal methods such as mass spectrometry can provide additional information not captured by this method.