LncRNA-H19 acts as a ceRNA to regulate HE4 expression by sponging miR-140 in human umbilical vein endothelial cells under hyperglycemia with or without α-Mangostin

Biomedicine & Pharmacotherapy

Yanli Luo¹  Youpeng Ling²  Wanjun Luo²  Zhao Fang³ 

[1] Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China;Department of Cardiovascular Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China;Department of Thoracic Surgery, Yixing Tumor Hospital, 45 East Dongshan Road, Yixing, Jiangsu Province, PR China;

关键词: Human umbilical vein endothelial cells;    High glucose;    α-Mangostin;    Long non-coding RNAs;    Competing endogenous RNA;    H19;   

DOI  :  

来源: DOAJ

【 摘 要 】

α-Mangostin play crucial role in several cellular progress, including hyperglycemia-induced inhibition of cell growth and promotion of cell apoptosis. Increasing evidence displayed the important roles of lncRNAs and their potential as novel targets for drug development in human disease. However, there is rare study to comprehensively and systematically explore the role and underlying mechanism of lncRNAs in human umbilical vein endothelial cells (HUVECs) under hyperglycemia with or without α-Mangostin. In this study, we firstly found that α-Mangostin reduced the high glucose-induced inhibition of cell proliferation and migration potential of HUVECs. Then, we performed RNA-seq to dissect the expression profiles of lncRNAs in HUVECs treated with high glucose or high glucose supplemented with α-Mangostin. The results showed that the expression of H19 and HE4 was down-regulated by high glucose and further, α-Mangostin restored the high glucose-induced inhibition of H19 and HE4 expression. Further examination demonstrated that the modulation of the H19 and HE4 expression affected the function of α-Mangostin in hyperglycemia. In addition, H19 regulated HE4 expression via the modulation of the miR-140 expression. Finally, we showed that H19 exerted its function via the modulation of H19/miR-140/HE4 in hyperglycemia with α-Mangostin. In summary, this study is the first to comprehensively identify the lncRNAs/mRNAs network in hyperglycemia with or without α-Mangostin, highlighting a novel regulatory pathway in hyperglycemia with or without α-Mangostin and indicating the potential therapeutic role of α-Mangostin in diabetes mellitus.

【 授权许可】

Unknown