Comparative Effect of Commercially Available Nanoparticles on Soil Bacterial Community and “Botrytis fabae” Caused Brown Spot: In vitro and in vivo Experiment

Frontiers in Microbiology

Carlos Adam Conte-Junior¹  Nader R. Abdelsalam²  Mayada A. Sabra²  Ahmed I. Heflish²  Mohamed T. El-Saadony³  Samyah D. Jastaniah⁴  Bothaina A. Alaidaroos⁴  Rehab Y. Ghareeb⁵  Marwa I. Mackled⁶ 

[1]Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, Brazil

[2]Department of Agricultural Botany, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria, Egypt

[3]Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, Egypt

[4]Department of Biological Science, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

[5]Department of Plant Protection and Biomolecular Diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt

[6]Department of Stored Product Pests, Agriculture Research Center (ARC), Plant Protection Institute, Sabahia, Alexandria, Egypt

关键词: microbial community;    fungi;    pathogens;    nanoparticles;    fungicide;    rhizosphere;   

DOI  :  10.3389/fmicb.2022.934031

来源: DOAJ

【 摘 要 】

This study revealed the possible effects of various levels of silver nanoparticle (AgNP) application on plant diseases and soil microbial diversity. It investigated the comparison between the application of AgNPs and two commercial nanoproducts (Zn and FeNPs) on the rhizobacterial population and Botrytis fabae. Two experiments were conducted. The first studied the influence of 13 AgNP concentration on soil bacterial diversity besides two other commercial nanoparticles, ZnNPs (2,000 ppm) and FeNPs (2,500 ppm), used for comparison and application on onion seedlings. The second experiment was designed to determine the antifungal activity of previous AgNP concentrations (150, 200, 250, 300, 400, and 500 ppm) against B. fabae, tested using commercial fungicide as control. The results obtained from both experiments revealed the positive impact of AgNPs on the microbial community, representing a decrease in both the soil microbial biomass and the growth of brown spot disease, affecting microbial community composition, including bacteria, fungi, and biological varieties. In contrast, the two commercial products displayed lower effects compared to AgNPs. This result clearly showed that the AgNPs strongly inhibited the plant pathogen B. fabae growth and development, decreasing the number of bacteria (cfu/ml) and reducing the rhizosphere. Using AgNPs as an antimicrobial agent in the agricultural domain is recommended.

【 授权许可】

Unknown