A Murine Model of Mycobacterium kansasii Infection Reproducing Necrotic Lung Pathology Reveals Considerable Heterogeneity in Virulence of Clinical Isolates

Frontiers in Microbiology

Eulógio C. Q. Carvalho¹  Thatiana L. B. V. Simão²  Vinicius O. Mussi²  Guilherme A. M. Sales²  Elena B. Lasunskaia²  Fabrício M. Almeida²  Sanderson D. Calixto²  Sidra E. G. Vasconcellos³  Edson Machado³  Philip N. Suffys³  Marcos Catanho⁴  Luciana D. de Carvalho⁵ 

[1] Laboratory of Animal Morphology and Pathology, State University of North Fluminense, Campos, Brazil;Laboratory of Biology of Recognition, State University of North Fluminense, Campos, Brazil;Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil;Laboratory of Molecular Genetics of Microorganisms, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil;National Reference Laboratory for Tuberculosis, Reference Center Professor Helio Fraga, National School of Public Health, Fiocruz, Rio de Janeiro, Brazil;

关键词: virulence;    animal models;    pulmonary disease;    clinical isolates;    Mycobacterium kansasii;    nontuberculous mycobacteria;   

DOI  :  10.3389/fmicb.2021.718477

来源: DOAJ

【 摘 要 】

Among non-tuberculous mycobacteria, Mycobacterium kansasii is one of the most pathogenic, able to cause pulmonary disease indistinguishable from tuberculosis in immunocompetent susceptible adults. The lack of animal models that reproduce human-like lung disease, associated with the necrotic lung pathology, impairs studies of M. kansasii virulence and pathogenicity. In this study, we examined the ability of the C57BL/6 mice, intratracheally infected with highly virulent M. kansasii strains, to produce a chronic infection and necrotic lung pathology. As a first approach, we evaluated ten M. kansasii strains isolated from Brazilian patients with pulmonary disease and the reference strain M. kansasii ATCC 12478 for virulence-associated features in macrophages infected in vitro; five of these strains differing in virulence were selected for in vivo analysis. Highly virulent isolates induced progressive lung disease in mice, forming large encapsulated caseous granulomas in later stages (120–150 days post-infection), while the low-virulent strain was cleared from the lungs by day 40. Two strains demonstrated increased virulence, causing premature death in the infected animals. These data demonstrate that C57BL/6 mice are an excellent candidate to investigate the virulence of M. kansasii isolates. We observed considerable heterogeneity in the virulence profile of these strains, in which the presence of highly virulent strains allowed us to establish a clinically relevant animal model. Comparing public genomic data between Brazilian isolates and isolates from other geographic regions worldwide demonstrated that at least some of the highly pathogenic strains isolated in Brazil display remarkable genomic similarities with the ATCC strain 12478 isolated in the United States 70 years ago (less than 100 SNPs of difference), as well as with some recent European clinical isolates. These data suggest that few pathogenic clones have been widely spread within M. kansasii population around the world.

【 授权许可】

Unknown