Recent Advances in Aptamer Sensors

Sensors

Dong-Hwan Kim¹  SamyM. Shaban¹ 

[1] School of Chemical Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea;

关键词: aptamer;    colorimetric aptasensor;    fluorometric aptasensor;    electrochemical aptasensor;   

DOI  :  10.3390/s21030979

来源: DOAJ

【 摘 要 】

Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

【 授权许可】

Unknown