Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection

Frontiers in Chemistry

Chemistry

Armelle Ringuedé¹  Yi Zou²  Cyrine Slim²  Fethi Bedioui²  Sophie Griveau²  Cyrille Richard³ 

[1] Institut de Recherche de Chimie de Paris (IRCP), PSL Research University, CNRS, Chimie ParisTech, Paris, France;Institute of Chemistry for Life and Health Sciences (i-CLeHS), Chimie ParisTech, PSL Research University, CNRS, Paris, France;Université de Paris, CNRS, INSERM, UTCBS, Paris, France;

关键词: electrochemical aptasensor;    aptamer;    functionalized multi-walled carbon nanotubes;    diclofenac;    impedimetric detection;   

DOI  :  10.3389/fchem.2021.812909

 received in 2021-11-10, accepted in 2021-12-23,  发布年份 2022

来源: Frontiers

【 摘 要 】

Driven by the increasing concern about the risk of diclofenac (DCF) residues as water pollutants in the aqueous environment and the growing need for its trace determination, a simple but sensitive electrochemical aptasensor for the trace detection of DCF was developed. To construct the aptasensor, the amine-terminated DCF aptamer was covalently immobilized on the surface of the carboxylic acid–functionalized multi-walled carbon nanotube (f-MWCNT)–modified glassy carbon electrode (GCE) through EDC/NHS chemistry. The f-MWCNTs provide a reliable matrix for aptamer immobilization with high grafting density, while the aptamer serves as a biorecognition probe for DCF. The obtained aptasensor was incubated with DCF solutions at different concentrations and was then investigated by electrochemical impedance spectroscopy (EIS). It displays two linear ranges of concentration for DCF detection, from 250 fM to 1pM and from 1 pM to 500 nM with an extremely low detection limit of 162 fM. Also, the developed biosensor shows great reproducibility, acceptable stability, and reliable selectivity. Therefore, it offers a simple but effective aptasensor construction strategy for trace detection of DCF and is anticipated to show great potential for environmental applications.

【 授权许可】

Unknown   
Copyright © 2022 Zou, Griveau, Ringuedé, Bedioui, Richard and Slim.