Vitamin D Deficiency Induces Elevated Oxidative and Biomechanical Damage in Coronary Arterioles in Male Rats

Antioxidants

Andrea Heinzlmann¹  Attila Magyar²  RékaEszter Sziva³  Zoltán Fontányi³  Szabolcs Várbíró³  EszterMária Horváth⁴  GyörgyL. Nádasy⁴  Rita Benkő⁴  Zoltán Benyó⁵  Anna Monori-Kiss⁵  Éva Pál⁵  Leila Hadjadj⁵ 

[1] Department of Anatomy and Histology, University of Veterinary Medicine, István Street 2, 1078 Budapest, Hungary;Department of Anatomy, Histology and Embriology, Semmelweis University, Tűzoltó Street 58, 1094 Budapest, Hungary;Department of Obstetrics and Gynaecology, Semmelweis University, Üllői Street 78/a, 1082 Budapest, Hungary;Department of Physiology, Semmelweis University, Tűzoltó Street 37-47, 1094 Budapest, Hungary;Institute of Translational Medicine, Semmelweis University, Tűzoltó Street 37-47, 1094 Budapest, Hungary;

关键词: vitamin D;    rat model;    cardiovascular disease;    oxidative damage;    vitamin D deficiency;    male;   

DOI  :  10.3390/antiox9100997

来源: DOAJ

【 摘 要 】

Background: Several reports prove interconnection between vitamin D (VD) deficiency and increased cardiovascular risk. Our aim was to investigate the effects of VD status on biomechanical and oxidative–nitrative (O–N) stress parameters of coronary arterioles in rats. Methods: 4-week-old male Wistar rats were divided into a control group (11 animals) with optimal VD supply (300 IU/kgbw/day) and a VD-deficient group (11 animals, <5 IU/kg/day). After 8 weeks, coronary arteriole segments were prepared. Geometrical, elastic, and biomechanical characteristics were measured by in vitro arteriography. O–N stress markers were investigated by immunohistochemistry. Results: Inner radius decreased; wall thickness and wall-thickness/lumen diameter ratio increased; tangential wall stress and elastic modulus were reduced in VD-deficient group. No difference could be found in wall-cross-sectional area, intima-media area %. While the elastic elements of the vessel wall decreased, the α-smooth muscle actin (α-SMA) immunostaining intensity showed no changes. Significant elevation was found in the lipid peroxidation marker of 4-hidroxy-2-nonenal (HNE), while other O–N stress markers staining intensity (poly(ADP)ribose, 3-nitrotyrosine) did not change. Conclusions: Inward eutrophic remodeling has developed. The potential background of these impairments may involve the initial change in oxidative damage markers (HNE). These mechanisms can contribute to the increased incidence of the cardiovascular diseases in VD deficiency.

【 授权许可】

Unknown