Biocementation by Sporosarcina pasteurii ATCC6453 under simulated conditions in sand columns

Journal of Materials Research and Technology

Abobaker Binyahya¹  Husain Abbas¹  Abdullah Almajed¹  Yousef Al-Salloum¹  Sarfaraz Hadi² 

[1] Chair of Research and Studies in Strengthening and Rehabilitation of Structures, Dept. of Civil Engineering, College of Engineering, King Saud University, P.O. Box 800, Riyadh, 11421, Saudi Arabia;Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, 11421, Saudi Arabia;

关键词: MICP;    Sand columns;    Sporosarcina pasteurii ATCC6453;    Urease;    SEM;    XRD;   

DOI  :  

来源: DOAJ

【 摘 要 】

The concept of microbially induced calcite precipitation (MICP) is in vogue for quite some time; but a multitude of factors that would lead to optimal bacterial activity enabling real time application of the technique in various intended processes are yet to be standardized. This study is an attempt to elucidate some of these factors. The study was conducted in sand columns simulating standard cement mortar conditions of high pH at room temperature. Sporosarcina pasteurii ATCC6453 cultures in broths at variable cell counts of 1 × 103, 105, 107, and 109 ml−1 with 0.2M concentrations of urea and calcium chloride were poured onto sand columns in plastic sheet molds. After variable incubation period of 24, 48, 72, and 96 h, urease activity of the bacterium was assayed calorimetrically. Physical verification of calcite deposition was done with scanning electron microscopy (SEM) and X-ray diffraction (XRD) after 28 days of treatment. Urease activity escalated significantly with increasing cell count and incubation period. SEM and XRD examinations revealed remarkable deposition of calcite in the columns under treatment.

【 授权许可】

Unknown