Multi-Component Antioxidative System and Robust Carbohydrate Status, the Essence of Plant Arsenic Tolerance

Antioxidants

Aneta Hrdinová¹  Helena Lipavská¹  Monika Kofroňová¹  Petra Mašková¹  Dominik Pinkas²  Jana Tremlová³  Šárka Petrová⁴  Petr Soudek⁴ 

[1] Department of Experimental Plant Biology, Faculty of Science, Charles University, Viničná 5, 2, 128 44 Prague, Czech Republic;Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 2, 128 44 Prague, Czech Republic;Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Science, Prague 6, Kamýcká, 961/129 Suchdol, Czech Republic;Laboratory of Plant Biotechnologies, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová, 313 Prague 6-Lysolaje, Czech Republic;

关键词: arsenate;    arsenic;    arsenite;    antioxidant;    antioxidant enzyme;    saccharides;   

DOI  :  10.3390/antiox9040283

来源: DOAJ

【 摘 要 】

Arsenic (As) contaminates the food chain and decreases agricultural production through impairing plants, particularly due to oxidative stress. To better understand the As tolerance mechanisms, two contrasting tobacco genotypes: As-sensitive Nicotiana sylvestris and As-tolerant N.tabacum, cv. ‘Wisconsin’ were analyzed. The most meaningful differences were found in the carbohydrate status, neglected so far in the As context. In the tolerant genotype, contrary to the sensitive one, net photosynthesis rates and saccharide levels were unaffected by As exposure. Importantly, the total antioxidant capacity was far stronger in the As-tolerant genotype, based on higher antioxidants levels (e.g., phenolics, ascorbate, glutathione) and activities and/or appropriate localizations of antioxidative enzymes, manifested as reverse root/shoot activities in the selected genotypes. Accordingly, malondialdehyde levels, a lipid peroxidation marker, increased only in sensitive tobacco, indicating efficient membrane protection in As-tolerant species. We bring new evidence of the orchestrated action of a broad spectrum of both antioxidant enzymes and molecules essential for As stress coping. For the first time, we propose robust carbohydrate metabolism based on undisturbed photosynthesis to be crucial not only for subsidizing C and energy for defense but also for participating in direct reactive oxygen species (ROS) quenching. The collected data and suggestions can serve as a basis for the selection of plant As phytoremediators or for targeted breeding of tolerant crops.

【 授权许可】

Unknown