Evaluation of Hepatoprotective Activity and Oxidative Stress Reduction of Rosmarinus officinalis L. Shoots Tincture in Rats with Experimentally Induced Hepatotoxicity

Molecules

Bogdan Sevastre¹  Raluca Marica¹  Mihaela Niculae²  Alina Uifălean³  Irina Ielciu⁴  Elisabeta Chișe⁵  Ilioara Oniga⁶  Daniela Hanganu⁶  Daniela Benedec⁶  AlexandraC. Sevastre-Berghian⁷  Andreea Turdean⁸  Neli-Kinga Olah⁸ 

[1] Department of Clinic and Paraclinic Sciences, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania;Department of Clinical Sciences, Division and Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, 400374 Cluj-Napoca, Romania;Department of Pharmaceutical Analysis, Faculty of Pharmacy, Iuliu Hațieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania;Department of Pharmaceutical Botany, Iuliu Haţieganu University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania;Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Vasile Goldiş Western University of Arad, 310414 Arad, Romania;Department of Pharmacognosy, Iuliu Haţieganu University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania;Department of Physiology, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania;PlantExtrakt, 407059 Cluj-Napoca, Romania;

关键词: Rosmarinus officinalis L.;    fresh young shoots tincture;    polyphenols;    terpenes;    antioxidant;    hepatoprotective;   

DOI  :  10.3390/molecules26061737

来源: DOAJ

【 摘 要 】

Rosmarinus officinalis L. is a widely known species for its medicinal uses, that is also used as raw material for the food and cosmetic industry. The aim of the present study was to offer a novel perspective on the medicinal product originating from this species and to test its hepatoprotective activity. The tested sample consisted in a tincture obtained from the fresh young shoots. Compounds that are evaluated for this activity are polyphenols and terpenoids, that are identified and quantified by HPLC–UV–MS and GC–MS. Antioxidant activity was assessed in vitro, using the DPPH, FRAP and SO assays. Hepatoprotective activity was tested in rats with experimentally-induced hepatotoxicity. In the chemical composition of the tincture, phenolic diterpenes (carnosic acid, carnosol, rosmanol, rosmadial) and rosmarinic acid were found to be the majority compounds, alongside with 1,8-cineole, camphene, linalool, borneol and terpineol among monoterpenes. In vitro, the tested tincture proved significant antioxidant capacity. Results of the in vivo experiment showed that hepatoprotective activity is based on an antioxidant mechanism. In this way, the present study offers a novel perspective on the medicinal uses of the species, proving significant amounts of polyphenols and terpenes in the composition of the fresh young shoots tincture, that has proved hepatoprotective activity through an antioxidant mechanism.

【 授权许可】

Unknown