Sounds Stimulation on In Vitro HL1 Cells: A Pilot Study and a Theoretical Physical Model

International Journal of Molecular Sciences

Francesco Tona¹  Carlo Dal Lin¹  Sabino Iliceto¹  Paolo Simioni²  Giuseppe Vitiello³  Paola Romano⁴  ClaudiaMaria Radu⁵  Albino Polcari⁶ 

[1] Department of Cardiac, Thoracic and Vascular Sciences, Padua University Medical School,35100 Padua, Italy;Department of Medicine, Thrombotic and Haemorrhagic Diseases Unit, Veneto Region Haemophilia and Thrombophilia Centre, University of Padua Medical School, 35100 Padua, Italy;Department of Physics “E.R.Caianiello”, Salerno University, Fisciano, 84084 Salerno, Italy;Department of Sciences and Technologies, Sannio University, Benevento, Italy;Department of Women’s and Children’s Health, University of Padua, 35100 Padua, Italy;IISS “Giovanni XXIII”, 84084 Salerno, Italy;

关键词: mechano-transduction;    cardiomyocytes;    cytoskeletal proteins;    sound waves;    coherent states;    fractals;   

DOI  :  10.3390/ijms22010156

来源: DOAJ

【 摘 要 】

Mechanical vibrations seem to affect the behaviour of different cell types and the functions of different organs. Pressure waves, including acoustic waves (sounds), could affect cytoskeletal molecules via coherent changes in their spatial organization and mechano-transduction signalling. We analyzed the sounds spectra and their fractal features. Cardiac muscle HL1 cells were exposed to different sounds, were stained for cytoskeletal markers (phalloidin, beta-actin, alpha-tubulin, alpha-actinin-1), and studied with multifractal analysis (using FracLac for ImageJ). A single cell was live-imaged and its dynamic contractility changes in response to each different sound were analysed (using Musclemotion for ImageJ). Different sound stimuli seem to influence the contractility and the spatial organization of HL1 cells, resulting in a different localization and fluorescence emission of cytoskeletal proteins. Since the cellular behaviour seems to correlate with the fractal structure of the sound used, we speculate that it can influence the cells by virtue of the different sound waves’ geometric properties that we have photographed and filmed. A theoretical physical model is proposed to explain our results, based on the coherent molecular dynamics. We stress the role of the systemic view in the understanding of the biological activity.

【 授权许可】

Unknown