Application of Force to a Syndecan-4 Containing Complex With Thy-1–αVβ3 Integrin Accelerates Neurite Retraction

Frontiers in Molecular Biosciences

Lisette Leyton¹  Samuel Martínez-Meza¹  Andrew F. G. Quest¹  Francesca Burgos-Bravo³  Christian A. M. Wilson³ 

[1] Advanced Center for Chronic Diseases, Facultad de Medicina, Universidad de Chile, Santiago, Chile;Laboratory of Cellular Communication, Center for Studies on Exercise, Metabolism and Cancer, Institute of Biomedical Sciences, Santiago, Chile;Single Molecule Biochemistry and Mechanobiology Laboratory, Department of Biochemistry and Molecular Biology, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile;

关键词: cell–cell adhesion;    single-molecule analysis;    trimolecular adhesion complex;    mechano-sensor;    mechano-transduction;    inflammation;   

DOI  :  10.3389/fmolb.2020.582257

来源: DOAJ

【 摘 要 】

Inflammation contributes to the genesis and progression of chronic diseases, such as cancer and neurodegeneration. Upregulation of integrins in astrocytes during inflammation induces neurite retraction by binding to the neuronal protein Thy-1, also known as CD90. Additionally, Thy-1 alters astrocyte contractility and movement by binding to the mechano-sensors αVβ3 integrin and Syndecan-4. However, the contribution of Syndecan-4 to neurite shortening following Thy-1–αVβ3 integrin interaction remains unknown. To further characterize the contribution of Syndecan-4 in Thy-1-dependent neurite outgrowth inhibition and neurite retraction, cell-based assays under pro-inflammatory conditions were performed. In addition, using Optical Tweezers, we studied single-molecule binding properties between these proteins, and their mechanical responses. Syndecan-4 increased the lifetime of Thy-1–αVβ3 integrin binding by interacting directly with Thy-1 and forming a ternary complex (Thy-1–αVβ3 integrin + Syndecan-4). Under in vitro-generated pro-inflammatory conditions, Syndecan-4 accelerated the effect of integrin–engaged Thy-1 by forming this ternary complex, leading to faster neurite retraction and the inhibition of neurite outgrowth. Thus, Syndecan-4 controls neurite cytoskeleton contractility by modulating αVβ3 integrin mechano-receptor function. These results suggest that mechano-transduction, cell-matrix and cell-cell interactions are likely critical events in inflammation-related disease development.

【 授权许可】

Unknown