Identification of Proteins Modulated in the Date Palm Stem Infested with Red Palm Weevil (Rhynchophorus ferrugineus Oliv.) Using Two Dimensional Differential Gel Electrophoresis and Mass Spectrometry

International Journal of Molecular Sciences

Muhammad Mukhtar¹  Muhammad Altaf Khan²  Muhammad Tufail³  Khawaja Ghulam Rasool³  Abdulrahman Saad Aldawood³  Makio Takeda⁴ 

[1] Department of Biotechnology, American University of Ras Al Khaimah, Ras Al Khaimah 10021, United Arab Emirates;Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia;Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia;Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan;

关键词: date palm;    stem;    red palm weevil;    infestation;    differential expression;    proteins;    two dimensional differential in-gel electrophoresis (2D-DIGE);    Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF);   

DOI  :  10.3390/ijms160819326

来源: DOAJ

【 摘 要 】

A state of the art proteomic methodology using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF) has been employed to characterize peptides modulated in the date palm stem subsequent to infestation with red palm weevil (RPW). Our analyses revealed 32 differentially expressed peptides associated with RPW infestation in date palm stem. To identify RPW infestation associated peptides (I), artificially wounded plants (W) were used as additional control beside uninfested plants, a conventional control (C). A constant unique pattern of differential expression in infested (I), wounded (W) stem samples compared to control (C) was observed. The upregulated proteins showed relative fold intensity in order of I > W and downregulated spots trend as W > I, a quite interesting pattern. This study also reveals that artificially wounding of date palm stem affects almost the same proteins as infestation; however, relative intensity is quite lower than in infested samples both in up and downregulated spots. All 32 differentially expressed spots were subjected to MALDI-TOF analysis for their identification and we were able to match 21 proteins in the already existing databases. Relatively significant modulated expression pattern of a number of peptides in infested plants predicts the possibility of developinga quick and reliable molecular methodology for detecting plants infested with date palm.

【 授权许可】

Unknown