International Journal of Molecular Sciences | |
Identification of Proteins Modulated in the Date Palm Stem Infested with Red Palm Weevil ( |
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Khawaja Ghulam Rasool1  Muhammad Altaf Khan4  Abdulrahman Saad Aldawood1  Muhammad Tufail1  Muhammad Mukhtar2  Makio Takeda3  | |
[1] Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia; E-Mails:;Department of Biotechnology, American University of Ras Al Khaimah, Ras Al Khaimah 10021, United Arab Emirates;Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan; E-Mail:;Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia; E-Mail: | |
关键词: date palm; stem; red palm weevil; infestation; differential expression; proteins; two dimensional differential in-gel electrophoresis (2D-DIGE); Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF); | |
DOI : 10.3390/ijms160819326 | |
来源: mdpi | |
【 摘 要 】
A state of the art proteomic methodology using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF) has been employed to characterize peptides modulated in the date palm stem subsequent to infestation with red palm weevil (RPW). Our analyses revealed 32 differentially expressed peptides associated with RPW infestation in date palm stem. To identify RPW infestation associated peptides (I), artificially wounded plants (W) were used as additional control beside uninfested plants, a conventional control (C). A constant unique pattern of differential expression in infested (I), wounded (W) stem samples compared to control (C) was observed. The upregulated proteins showed relative fold intensity in order of I > W and downregulated spots trend as W > I, a quite interesting pattern. This study also reveals that artificially wounding of date palm stem affects almost the same proteins as infestation; however, relative intensity is quite lower than in infested samples both in up and downregulated spots. All 32 differentially expressed spots were subjected to MALDI-TOF analysis for their identification and we were able to match 21 proteins in the already existing databases. Relatively significant modulated expression pattern of a number of peptides in infested plants predicts the possibility of developing a quick and reliable molecular methodology for detecting plants infested with date palm.
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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