3D Printed Lab-on-a-Chip Platform for Chemical Stimulation and Parallel Analysis of Ion Channel Function

Micromachines

Daniel Aschenbrenner¹  Oliver Friedrich¹  DanielF. Gilbert¹ 

[1] Institute of Medical Biotechnology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91052 Erlangen, Germany;

关键词: 3D printing;    ABS;    microfluidic;    zigzag channel;    Ca²⁺ imaging;    Fluo-4 AM;    TRPV1;    capsaicin;    HEK293 cells;   

DOI  :  10.3390/mi10080548

来源: DOAJ

【 摘 要 】

Functional imaging has been a widely established method for the assessment of ion channel function in vitro. Conventional infrastructure used for in vitro functional analysis of ion channels is typically proprietary, non-customizable, expensive, and requires a high level of skill to use and maintain. 3D desktop printing, which is employed in the rapid prototyping field, allows for quick engineering of alternatives to conventional imaging infrastructure that are customizable, low cost, and user friendly. Here, we describe an ultra-low-cost microfluidic lab-on-a-chip (LOC) device manufactured using acrylonitrile butadiene styrene (ABS) for in vitro functional imaging of ion channels that can quickly and easily be reconstructed using three-dimensional (3D) desktop printing. The device is light weight (<5 g), small (20 mm × 49 mm), and extremely low cost (2+ imaging with TRPV1 channels, the device may also be useful for application with other ion channel types and in vitro models.

【 授权许可】

Unknown