Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux

Nutrients

Xuezhi Zuo¹  Piwei Zhang¹  Miying Du²  Chenjiang Ying³  Xiao Xie³  Weijie Yi³  Nannan Wu³  Siyun Xiang³  Eskedar Getachew Assefa³  Hui Yang⁴  Chong Tian⁵  Qiaoqiao Yan⁶ 

[1] Department of Clinical Nutrition, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China;Department of Hotel Management, Tourism University, Guilin 541006, Guangxi, China;Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China;Kecheng People’s Hospital, Quzhou 324000, Zhejiang, China;School of Nursing, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China;School of Public Health and Management, Wenzhou Medical University, Wenzhou 325003, Zhejiang, China;

关键词: green tea polyphenols;    renal function;    autophagy;    high-fat diet;    dietary restriction;   

DOI  :  10.3390/nu9050497

来源: DOAJ

【 摘 要 】

Epidemiological and experimental studies reveal that Western dietary patterns contribute to chronic kidney disease, whereas dietary restriction (DR) or dietary polyphenols such as green tea polyphenols (GTPs) can ameliorate the progression of kidney injury. This study aimed to investigate the renal protective effects of GTPs and explore the underlying mechanisms. Sixty Wistar rats were randomly divided into 6 groups: standard diet (STD), DR, high-fat diet (HFD), and three diets plus 200 mg/kg(bw)/day GTPs, respectively. After 18 weeks, HFD group exhibited renal injuries by increased serum cystatin C levels and urinary N-acetyl-β-d-glucosaminidase activity, which can be ameliorated by GTPs. Meanwhile, autophagy impairment as denoted by autophagy-lysosome related proteins, including LC3-II, Beclin-1, p62, cathepsin B, cathepsin D and LAMP-1, was observed in HFD group, whereas DR or GTPs promoted renal autophagy activities and GTPs ameliorated HFD-induced autophagy impairment. In vitro, autophagy flux suppression was detected in palmitic acid (PA)-treated human proximal tubular epithelial cells (HK-2), which was ameliorated by epigallocatechin-3-gallate (EGCG). Furthermore, GTPs (or EGCG) elevated phosphorylation of AMP-activated protein kinase in the kidneys of HFD-treated rats and in PA-treated HK-2 cells. These findings revealed that GTPs mimic the effects of DR to induce autophagy and exert a renal protective effect by alleviating HFD-induced autophagy suppression.

【 授权许可】

Unknown