Differential Effects of Mycobacterium bovis BCG on Macrophages and Dendritic Cells from Murine Spleen

International Journal of Molecular Sciences

Zhiming Pan¹  Xiang Chen¹  Chuang Meng¹  Bin Qiang¹  Yuelan Yin¹  Lin Sun¹  Xinan Jiao¹  Hongyan Gu¹  Zhengzhong Xu¹ 

[1] Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention andControl of Important Animal Infectious Diseases and Zoonoses, Yangzhou University,Yangzhou 225009, China;

关键词: BCG;    macrophage;    dendritic cell;   

DOI  :  10.3390/ijms161024127

来源: DOAJ

【 摘 要 】

Macrophages (MΦ) and dendritic cells (DCs) are both pivotal antigen presenting cells capable of inducing specific cellular responses to inhaled mycobacteria, and thus,they may be important in the initiation of early immune responses to mycobacterial infection.In this study, we evaluated and compared the roles of murine splenic DCs and MΦs inimmunity against Mycobacterium bovis Bacillus Calmette-Guérin (M.bovis BCG). The number of internalized rBCG-GFP observed was obviously greater in murine splenic MΦs compared with DCs, and the intracellular reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in MΦs were all higher than in DCs. DCs havea stronger capacity for presenting Ag85A peptide to specific T hybridoma and when themurine splenic MΦs were infected with BCG and rBCG::Ag85A, low level of antigen presenting activity was detected. These data suggest that murine splenic MΦs participate in mycobacteria uptake, killing and inducing inflammatory response, whereas the murine splenic DCs are primarily involved in specific antigen presentation and T cell activation.

【 授权许可】

Unknown