Brain Ischemia Significantly Alters microRNA Expression in Human Peripheral Blood Natural Killer Cells

Frontiers in Immunology

Hongshan Ma¹  Honglei Ren²  Bohao Zhang³  Xiao-An Zhang³  Xiaojing Cheng⁵  Minshu Li⁵  Shiyao Li⁵  Ying Kong⁵ 

[1] Center for Neurological Diseases, The Third People's Hospital of Datong, Datong, China;Department of Anesthesiology and Critical Care Medicine, Johns Hopkins School of Medicine, Baltimore, MD, United States;Department of Imaging, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China;Department of Neurology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin, China;Tianjin Neurological Institute, Key Laboratory of Post-neurotrauma Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin, China;

关键词: stroke;    natural killer cells;    immunosuppression;    molecular regulation;    microRNA;   

DOI  :  10.3389/fimmu.2020.00759

来源: DOAJ

【 摘 要 】

Brain ischemia induces systemic immunosuppression and increases a host's susceptibility to infection. MicroRNAs (miRNAs) are molecular switches in immune cells, but the alterations of miRNAs in human immune cells in response to brain ischemia and their impact on immune defense remain elusive. Natural killer (NK) cells are critical for early host defenses against pathogens. In this study, we identified reduced counts, cytokine production, and cytotoxicity in human peripheral blood NK cells obtained from patients with acute ischemic stroke. The extent of NK cell loss of number and activity was associated with infarct volume. MicroRNA sequencing analysis revealed that brain ischemia significantly altered miRNA expression profiles in circulating NK cells, in which miRNA-451a and miRNA-122-5p were dramatically upregulated. Importantly, inhibition of miR-451a or miR-122-5p augmented the expression of activation-associated receptors in NK cells. These results provide the first evidence that brain ischemia alters miRNA signatures in human NK cells.

【 授权许可】

Unknown