Molecular Epidemiology of Babesia and Theileria Species in Sheep in Kars Region of Turkey

Türkiye Parazitoloji Dergisi

Mükremin Özkan Arslan¹  Zati Vatansever²  Nilgün Aydın² 

[1] Kafkas University Faculty of Medicine, Department of Medical Parasitology, Kars, Turkey;Kafkas University Faculty of Veterinary Medicine, Department of Parasitology, Kars, Turkey;

关键词: babesia;    theileria;    reverse line blotting;    sheep;    turkey;   

DOI  :  10.4274/tpd.galenos.2021.09709

来源: DOAJ

【 摘 要 】

Objective:This study aimed to evaluate Babesia and Theileria species and vector ticks in sheep in the Eastern Anatolia Region of Turkey.Methods:Blood samples were collected from 960 sheep, and ticks were collected from the same animals between January and December 2017. The reverse line blotting (RLB) method was used to analyze Babesia and Theileria piroplasm DNAs. Ticks and tick egg clusters were evaluated in terms of Babesia and Theileria species using the RLB technique.Results:Microscopically, 3.96% (38/960) of Theileria spp. piroplasm forms were identified; however, no Babesia spp. piroplasm forms were identified. The distribution of Babesia and Theileria spp. by RLB was 35.52% (341/960). The species identified included Theileria ovis (24.79%, 238/960), Theileria sp. (6.15%, 59/960), Theileria sp. OT3 (4.27%, 41/960), and Babesia ovis (0.31%, 3/960). Tick infestation was found in 17.5% (168/960) of the sheep. Dermacentor marginatus (66.31%), Haemaphysalis parva (32.73%), Hae. punctata (0.21%), Rhipicephalus bursa (0.53%), and Hyalomma marginatum (0.11%) were identified in the infected sheep. No pathogenic species were found in the analysis of egg clusters or tick carcasses according to the RLB method.Conclusion:Theileria ovis is the theileriosis agent in sheep in the study region. Species commonly detected in tick-infested sheep were D. marginatus and Hae. parva.

【 授权许可】

Unknown