Pyrosequencing investigation into the influence of Cu2+, Zn2+, Fe2+ and I− mixtures on fungal diversity and toxigenic fungal growth in a fermented liquid feed

Animal Nutrition

Huadong Wu¹  Zhiyu Chen²  Wei Lu²  Yuyong He²  Hong Wen³ 

[1] College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China;Jiangxi Provincial Institute of Veterinary Drugs and Feed Control, Nanchang 330096, China;

关键词: Trace elements;    Fungal growth;    Mixture of ions;    Fermented liquid feed;    Pyrosequencing;   

DOI  :  10.1016/j.aninu.2016.02.004

来源: DOAJ

【 摘 要 】

A L9(34) orthogonal experiment was conducted to evaluate the influence of 9 mixtures which consisted of Cu2+, Zn2+, Fe2+ and I− ions at different ion concentrations on fungal diversity and toxigenic fungal growth in a Bacillus subtilis-fermented liquid feed (FLF) using pyrosequencing. The maximal Chao estimator and Shannon index were achieved in the FLF with a mixture of Cu2+ (200 mg/kg), Zn2+ (160 mg/kg), Fe2+ (150 mg/kg) and I− (2.4 mg/kg). The minimal relative abundance of Aspergillus was achieved when a mixture of Cu2+ (200 mg/kg), Zn2+, Fe2+ and I− was added to the FLF. Compared with Zn2+, Fe2+ and I−, Cu2+ was the most important ion in inhibiting Aspergillus growth. Adding Zn2+ (160 mg/kg), Cu2+, Fe2+ and I− to the FLF minimized the relative abundance of Fusarium. It was Zn2+ instead of Cu2+ played a critical role in suppressing the growth of Fusarium. The proper use of the mixture of Cu2+, Zn2+, Fe2+ and I− in FLF contributes to inhibit the growth of mycotoxin-producing fungi during storage. The new findings of this study help farmers properly use the mixture of Cu2+, Zn2+, Fe2+ and I− to inhibit the growth of mycotoxin-producing fungi in the production of high quality FLF and alleviate mycotoxins damages to animals and humans.

【 授权许可】

Unknown