Manifold passages in an assorted infection in a host could improve virulence of Helicoverpa armigera Nucleopolyhedrovirus (HaNPV)

Saudi Journal of Biological Sciences

Muhammad Sohail Shahzad¹  Shafqat Saeed²  Muhammad Ali³  Khalid Ali Khan⁴  Syed Muhammad Zaka⁵  Allah Ditta Abid⁵  Naeem Iqbal⁶ 

[1] Corresponding author at: Institute of Plant Protection, MNS-University of Agriculture, Multan (60000), Pakistan;Department of Plant Protection, Karachi Ministry of National Food Security, Pakistan;Institute of Plant Protection, MNS-University of Agriculture, Multan (60000), Pakistan;Department of Entomology, Bahauddin Zakariya University, Multan (60800), Pakistan;Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan;Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan (60800), Pakistan;

关键词: American bollworm;    NPV;    Lethal time;    Toxicity;    Vertical transmission;   

DOI  :  

来源: DOAJ

【 摘 要 】

Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is serious pests of cotton and several other crops. Helicoverpa armigera Nucleopolyhedrovirus (HaNPV) can be important alternative to synthetic insecticides for the management of H. armigera. However, the efficacy of HaNPV can vary in horizontal and vertical transmission. In the current study, we evaluated the efficacy of HaNPV of a virulent strain (vertically transmitted up to six generations) and wild strains (used after isolation from the field infected larvae). Both strains were applied to the 2nd instar larvae of H. armigera @ 1 × 109 polyhedral inclusion bodies (PIB)/ml. There were six replications of each strain (strains). The results indicated higher mortalities in larvae exposed to virulent strains (68.33 ± 6.07%) as compared to wild strain (45 ± 2.24%). Virulent strains killed the larvae quite faster than wild strain. The lethal time (LT50) to kill 50% of the larvae by virulent strain was 7.15 days and for wild strain it was 19.47 days. The results showed that multiple passage of HaNPV through several generations enhances its efficacy to kill H. armigera larvae faster. The results of this study will be helpful to manage H. armigera and other related lepidopoterous pests.

【 授权许可】

Unknown