Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways

International Journal of Molecular Sciences

Ewa Mlyczyńska¹  Agnieszka Rak¹  Patrycja Kurowska¹  Katarzyna Poniedziałek-Kempny²  Katarzyna Soból²  Iwona Rajska²  Anthony Estienne³  Joelle Dupont³  Alix Barbe³ 

[1] Department of Physiology and Toxicology of Reproduction, Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, 30-387 Krakow, Poland;Department of Reproductive Biotechnology and Cryopreservation, National Research Institute of Animal Production, 32-083 Balice, Poland;INRAE, UMR85, Unité Physiologie de la Reproduction et des Comportements, 37380 Nouzilly, France;

关键词: oocyte maturation;    vaspin;    ovary;    adipokines;    MAP3/1;    PRKAA1;   

DOI  :  10.3390/ijms21249342

来源: DOAJ

【 摘 要 】

Oocyte maturation is a critical stage in embryo production and female reproduction. The aims of this study were to determine: (i) the mRNA and protein expression of vaspin and its receptor 78-kDa glucose-regulated (GRP78) in porcine cumulus–oocyte complexes (COCs) by real-time PCR and Western blot analysis, respectively, and their localisation by immunofluorescence; and (ii) the effects of vaspin on in vitro oocyte maturation (IVM) and the involvement of mitogen ERK1/2 (MAP3/1)- and AMPKα (PRKAA1)-activated kinases in the studied processes. Porcine COCs were matured in vitro for 22 h or 44 h with vaspin at a dose of 1 ng/mL and nuclear maturation assessed by Hoechst 33342 or DAPI staining and the measurement of progesterone (P4) level in the maturation medium. We showed that vaspin and GRP78 protein expression increased in oocytes and cumulus cells after IVM. Moreover, vaspin enhanced significantly porcine oocyte IVM and P4 concentration, as well as MAP3/1 phosphorylation, while decreasing PRKAA1. Using pharmacological inhibitors of MAP3/1 (PD98059) and PRKAA1 (Compound C), we observed that the effect of vaspin was reversed to the control level by all studied parameters. In conclusion, vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

【 授权许可】

Unknown