The effect of fluoroquinolones and antioxidans on biofilm formation by Proteus mirabilis strains

Annals of Clinical Microbiology and Antimicrobials

Ewa Wałecka-Zacharska¹  Eugenia Gospodarek-Komkowska²  Joanna Kwiecińska-Piróg²  Jana Przekwas²  Krzysztof Skowron²  Natalia Wiktorczyk-Kapischke²  Jakub Gębalski³  Dorota Rutkowska⁴ 

[1] Department of Food Hygiene and Consumer Health, Wrocław University of Environmental and Life Sciences;Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz;Department of Pharmaceutical Botany and Pharmacognosy, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz;Medical Education Unit, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz;

关键词: Biofilm;    Proteus mirabilis;    Antioxidants;    Antibiotics;   

DOI  :  10.1186/s12941-022-00515-5

来源: DOAJ

【 摘 要 】

Abstract Background Fluoroquinolones are a group of antibiotics used in urinary tract infections. Unfortunately, resistance to this group of drugs is currently growing. The combined action of fluoroquinolones and other antibacterial and anti-biofilm substances may extend the use of this therapeutic option by clinicians. The aim of the study was to determine the effect of selected fluoroquinolones and therapeutic concentrations of ascorbic acid and rutoside on biofilm formation by Proteus mirabilis. Materials and methods The study included 15 strains of P. mirabilis isolated from urinary tract infections in patients of the University Hospital No. 1 dr A. Jurasz in Bydgoszcz (Poland). The metabolic activity of the biofilm treated with 0.4 mg/ml ascorbic acid, 0.02 µg/ml rutoside and chemotherapeutic agents (ciprofloxacin, norfloxacin) in the concentration range of 0.125–4.0 MIC (minimum inhibitory concentration) was assessed spectrophotometrically. Results Both ciprofloxacin and norfloxacin inhibited biofilm formation by the tested strains. The biofilm reduction rate was correlated with the increasing concentration of antibiotic used. No synergism in fluoroquinolones with ascorbic acid, rutoside or both was found. The ascorbic acid and rutoside combination, however, significantly decreased biofilm production. Conclusions Our research proves a beneficial impact of ascorbic acid with rutoside supplementation on biofilm of P. mirabilis strains causing urinary tract infections.

【 授权许可】

Unknown