Cadmium-Tolerant Plant Growth-Promoting Bacteria Curtobacterium oceanosedimentum Improves Growth Attributes and Strengthens Antioxidant System in Chili (Capsicum frutescens)

Sustainability

Kartik Patel¹  Mitesh Patel¹  Md Imtaiyaz Hassan²  Dharmendra Kumar Yadav³  Lamya Ahmed Al-Keridis⁴  Nawaf Alshammari⁵  Riadh Badraoui⁵  Mohd Adnan⁵  Abdelbaset Mohamed Elasbali⁶  Waleed Abu Al-Soud⁷ 

[1] Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat 395007, India;Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India;College of Pharmacy, Gachon University of Medicine and Science, Hambakmoeiro, Yeonsu-gu, Incheon City 21924, Korea;Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh P.O. Box 84428, Saudi Arabia;Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia;Department of Clinical Laboratory Science, College of Applied Sciences-Qurayyat, Jouf University, Al-Jouf P.O. Box 2014, Saudi Arabia;Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia;

关键词: cadmium;    biosorption;    bioremediation;    Curtobacterium oceanosedimentum;    phytostabilization;    Chili;   

DOI  :  10.3390/su14074335

来源: DOAJ

【 摘 要 】

The remediation of potentially toxic element-polluted soils can be accomplished through the use of microbial and plant-assisted bioremediation. A total of 32 bacteria were isolated from soil samples contaminated with potentially toxic elements. The isolated bacterial strain DG-20 showed high tolerance to cadmium (up to 18 mM) and also showed bioaccumulative Cd removal properties, as demonstrated by atomic absorption spectroscopy studies. By sequencing the 16S rRNA gene, this strain was identified as Curtobacterium oceanosedimentum. Under stress and normal conditions, isolate DG-20 also produced a wide range of plant growth promoting traits, including ammonia production (51–73 µg/mL) and IAA production (116–183 µg/mL), alongside siderophore production and phosphate solubilization. Additionally, pot experiments were conducted to determine whether the strain could promote Chili growth when Cd salts are present. Over the control, bacterial colonization increased root and shoot lengths significantly up to 58% and 60%, respectively. Following inoculation with the Cd-tolerant strain, the plants also increased in both fresh and dry weight. In both the control and inoculated plants, Cd was accumulated more in roots than in shoots, indicating that Chili was phytostabilizing Cd levels. Besides improving the plant attributes, Cd-tolerant bacteria were also found to increase the amount of total chlorophyll, proline, total phenol, and ascorbic acid in the soil when added to the soil. These results suggest that the inoculant provides protection to plants from negative effects. The results of the present study predict that the combined properties of the tested strain in terms of Cd tolerance and plant growth promotion can be exploited for the purpose of the bioremediation of Cd, and for the improvement of Chili cultivation in soils contaminated with Cd.

【 授权许可】

Unknown