Effects of Exercise Training on the Autophagy-Related Muscular Proteins Expression in Ovariectomized Rats

Frontiers in Physiology

Huimin Bu¹  Xiangrong Shi²  Lianlian Wu³  Honghua Yuan⁴  Renwei Wang⁵  Weiquan Zhong⁶ 

[1] Department of Physiology, Xuzhou Medical University, Xuzhou, China;Institute for Healthy Aging, University of North Texas Health Science Center, Fort Worth, TX, United States;Laboratory Animal Center, Xuzhou Medical University, Xuzhou, China;Research Facility Center for Morphology, Xuzhou Medical University, Xuzhou, China;School of Kinesiology, Shanghai University of Sport, Shanghai, China;School of Medical Technology, Xuzhou Medical University, Xuzhou, China;

关键词: autophagy;    body weight;    ovariectomy;    skeletal muscle;    swimming exercise;   

DOI  :  10.3389/fphys.2019.00735

来源: DOAJ

【 摘 要 】

Ovariectomy disrupts estrogen production and homeostasis. However, whether exercise training (ET) could counteract the ovariectomy-induced effect on muscular autophagy has remained elusive. This study examined muscular autophagy in ovariectomized (OVX) rats following 8 weeks of swimming ET. Here, 40 6-month-old female Sprague-Dawley rats were randomly divided into five groups: sham-operated control (Sham), OVX control (OVX), OVX with 60-min ET (OVX-60ET), 90-min ET (OVX-90ET), and 120-min ET (OVX-120ET) for 6 days/week. According to the results of Western blotting, the expression levels of autophagy-related proteins in the OVX gastrocnemius muscle, including mammalian target of rapamycin, uncoordinated 51-like kinase 1, Beclin-1, autophagy-related gene (Atg-7), and microtubule-associated protein light chains 3 were significantly decreased (all P < 0.05), while there was an elevation on the p62 level. ET appreciably mitigated the OVX-induced negative effects on muscle quality and the autophagy pathway, which seemed to be dependent on ET volume. The most optimal outcomes were observed in the OVX-90ET group. The OVX-120 group had an adversely augmented catabolic process associated with gastrocnemius muscle atrophy. In conclusion, the expression levels of autophagy proteins are decreased in OVX rats, which can be appreciably mitigated following 8 weeks of swimming ET.

【 授权许可】

Unknown