Isoelectric Point Separations of Peptides and Proteins

Proteomes

Stephanie M. Cologna¹  Melissa R. Pergande¹ 

[1] Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607, USA;

关键词: isoelectric focusing;    proteomics;    electrophoresis;    two-dimensional gel electrophoresis;    isoelectric trapping;    capillary isoelectric focusing;   

DOI  :  10.3390/proteomes5010004

来源: DOAJ

【 摘 要 】

The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also discussed including common detection methods used. Applications in a variety of fields using isoelectric point based separations are provided as well as an outlook on the field for future studies.

【 授权许可】

Unknown