Proteomics analysis after traumatic brain injury in rats: the search for potential biomarkers

Arquivos de Neuro-Psiquiatria

Jun Ding¹  Zhengang Ding¹  Fang Yuan¹  Jianyi Guo¹  Hao Chen¹  Wenwei Gao¹  Ren Wang¹  Yi Gu¹  Jiong Chen¹  Yan Guo¹  Hengli Tian¹ 

关键词: traumatic brain injury;    biomarkers;    proteomics;    two-dimensional gel electrophoresis;    mass spectrometry;    lesão traumática cerebral;    biomarcadores;    proteômica;    eletroforese bidimensional em gel;    espectrometria de massa;   

DOI  :  10.1590/0004-282X20150006

来源: SciELO

【 摘 要 】

Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. After proteomic analysis, we detected 405 and 371 protein spots within a pH range of 3-10 from sham-treated and contused brain cortex, respectively. Eighty protein spots were differentially expressed in the two groups and 20 of these proteins were identified. This study validated the established biomarkers of TBI and identified potential biomarkers that could be examined in future work.

【 授权许可】

CC BY   
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