Human Hair Follicle-Derived Mesenchymal Stromal Cells from the Lower Dermal Sheath as a Competitive Alternative for Immunomodulation

Biomedicines

Andoni Cuevas¹  Manoli Igartua²  Ainhoa Gonzalez-Pujana²  Rosa Maria Hernandez²  Edorta Santos-Vizcaino²  Kara L. Spiller³  Beatriz Hernaez-Estrada³  Ander Izeta⁴ 

[1] Clínica Ercilla, 48009 Bilbao, Spain;NanoBioCel Research Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain;School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA 19104, USA;Tissue Engineering Group, Biodonostia Health Research Institute, 20014 Donostia-San Sebastián, Spain;

关键词: immunomodulation;    hair follicle;    MSCs;    macrophages;    regulatory T cells;    PBMC;   

DOI  :  10.3390/biomedicines10020253

来源: DOAJ

【 摘 要 】

Mesenchymal stromal cells (MSCs) have unique immunomodulatory capacities. We investigated hair follicle-derived MSCs (HF-MSCs) from the dermal sheath, which are advantageous as an alternative source because of their relatively painless and minimally risky extraction procedure. These cells expressed neural markers upon isolation and maintained stemness for a minimum of 10 passages. Furthermore, HF-MSCs showed responsiveness to pro-inflammatory environments by expressing type-II major histocompatibility complex antigens (MHC)-II to a lesser extent than adipose tissue-derived MSCs (AT-MSCs). HF-MSCs effectively inhibited the proliferation of peripheral blood mononuclear cells equivalently to AT-MSCs. Additionally, HF-MSCs promoted the induction of CD4+CD25+FOXP3+ regulatory T cells to the same extent as AT-MSCs. Finally, HF-MSCs, more so than AT-MSCs, skewed M0 and M1 macrophages towards M2 phenotypes, with upregulation of typical M2 markers CD163 and CD206 and downregulation of M1 markers such as CD64, CD86, and MHC-II. Thus, we conclude that HF-MSCs are a promising source for immunomodulation.

【 授权许可】

Unknown