Expression profile of circular RNA and construction of circular RNA-Micro RNA network in salivary adenoid cystic carcinoma

Cancer Cell International

Chunye Zhang¹  Zhimin Xu²  Nannan Han³  Jing Han³  Min Ruan³  Jiannan Liu³ 

[1] Department of Oral Pathology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine;Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University;Departments of Oral and Maxillofacial-Head and Neck Oncology, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine;

关键词: Circular RNA;    Salivary adenoid cystic carcinoma;    microRNA;    RNA sequencing;    circRNA ABCA13;   

DOI  :  10.1186/s12935-020-01681-2

来源: DOAJ

【 摘 要 】

Abstract Background Circular RNAs (circRNAs) is a newly discovered type of non-coding RNA, the abnormal expression of which has been demonstrated in many types of human tumors. So they have been considered as promising candidates as diagnostic and therapeutic targets in cancer. This research aimed to screen the profile of circRNA expression in salivary adenoid cystic carcinoma (SACC). Methods Using the threshold of FDR < 0.05 and fold change > 2 or < 0.5, 5 up-regulated and 26 down-regulated circRNAs were identified. The reliability of sequencing was verified by the expression detection of randomly selected circRNAs via qRT-PCR. Results Moreover, the circRNA-miRNA system was established by bioinformatics approaches and successfully identified an interaction between circRNA ABCA13 and a cancer-related miRNA (miR-138-5p), which was also verified by qRT-PCR. Moreover, the predicted molecular interaction proved that circRNA ABCA13 may promote SACC through inhibition of miR-138-5p. Conclusions Collectively, this study has offered the first report about the circRNA expression profile and circRNA-miRNA network in SACC. All of the above could benefit the exploration of novel therapeutic target in SACC treatment.

【 授权许可】

Unknown