Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method

Biotechnology Reports

Siti Nur Nadhirah Said Azmi¹  Fathin Amila Faisul Aris²  Nurul Nabilah Khairul Anuar³  Jessica Harriette Supang Anak John³  Sharifah Soplah Syed Abdullah³  Ahmad Syafiq Fauzan Mohd Asnawi³ 

[1] Corresponding author.;Section of Food Engineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia;Section of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, Malaysia;

关键词: Bacterial cellulose;    Ciprofloxacin;    In situ incorporation;    Acetobacter xylinum;   

DOI  :  

来源: DOAJ

【 摘 要 】

This paper describes the interaction and properties of bacterial cellulose (BC)–ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, Acetobacter xylinum 0416, was first tested to determine its inhibitory activity against the bacteria. In situ incorporation method was performed by introducing 0.2% (w/v) ciprofloxacin into Hestrin–Schramm medium at the onset of exponential phase of A. xylinum 0416 growth. Following a 10-day incubation at 28 °C, BC–ciprofloxacin composites were harvested and further characterised, while another BC–ciprofloxacin composite was harvested and purified prior to characterisation. The interaction between ciprofloxacin and BC was proven by the presence of quinolines and fluorine groups of ciprofloxacin on unpurified BC–ciprofloxacin composite and the reduction of crystallinity index as compared to the native BC. Moreover, deposited ciprofloxacin crystals on BC film and its composition were exhibited via SEM-Energy-dispersive X-ray analysis. Unpurified BC–ciprofloxacin film was determined to have strongly inhibited the following selected diabetic foot ulcer bacteria: E. coli, K. pneumoniae and P. aeruginosa. BC has the potential to be used as a wound dressing and a carrier for ciprofloxacin.

【 授权许可】

Unknown