Amorphous Selenium Nanoparticles Improve Vascular Function in Rats With Chronic Isocarbophos Poisoning via Inhibiting the Apoptosis of Vascular Endothelial Cells

Frontiers in Bioengineering and Biotechnology

Yaling Yin¹  Moli Zhu²  Lin Yang²  Chaonan Zhu⁴  Yinan Wu⁵  Zhitao Gao⁶  Yue Qiu⁷  Tiantian Zhu⁷  Peng Li⁷  Yutian Fu⁷  Qianqian Wang⁷  Keke Huang⁷ 

[1] Basic Medical College, Xinxiang Medical University, Xinxiang, China;Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China;Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, China;Department of Pharmacy, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China;Sanquan Medical College, Xinxiang Medical University, Xinxiang, China;School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, China;School of Pharmacy, Xinxiang Medical University, Xinxiang, China;

关键词: amorphous selenium nanoparticles;    isocarbophos;    sodium hydrogen exchanger 1;    apoptosis;    human umbilical vein endothelial cells;    vascular dysfunction;   

DOI  :  10.3389/fbioe.2021.673327

来源: DOAJ

【 摘 要 】

AimThis study aimed to investigate the preventive effect and possible mechanism of amorphous selenium nanoparticles (A-SeQDs) on isocarbophos induced vascular dysfunction.MethodsA-SeQDs was made by auto redox decomposition of selenosulfate precursor. Male rats were given isocarbophos (0.5 mg/kg/2 days) by intragastric administration for 16 weeks to induce vascular dysfunction. During the course, A-SeQDs (50 mg/kg/day) was added to the water from week 5. Then, the rats were killed to observe and test the influence of A-SeQDs on the vascular dysfunction induced by isocarbophos. Finally, human umbilical vein endothelial cells (HUVECs) were treated with 10% DMEM of isocarbophos (100 μM) for 5 days to detect the related indexes. Before the use of isocarbophos treatment, different drugs were given.ResultsA-SeQDs could reduce total carbon dioxide, MDA, VCAM-1, ICAM-1, IL-1, and IL-6 while increasing oxygen saturation, NO content, and SOD activity in rats. A-SeQDs also resulted in relatively normal vascular morphology, and the expression of sodium hydrogen exchanger 1 (NHE1) and caspase-3 decreased in rats. Furthermore, in HUVECs treated with isocarbophos, A-SeQDs maintained mitochondrial membrane potential, inhibited the cleaved caspase-3 expression, and released cytochrome c from mitochondria to cytosol.ConclusionA-SeQDs can inhibit the apoptosis of HUVECs through the mitochondrial pathway, and effectively treat the impairment of vascular endothelial function caused by isocarbophos, which is NHE1-dependent.

【 授权许可】

Unknown