Genetic variation of common carp Cyprinus carpio L. in China based on mitochondrial COII gene

Aquaculture Reports

Xianhu Zheng¹  Youyi Kuang²  Xiaochen Zhu³  Yingying Zhao³  Xiaowen Sun³ 

[1] Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China;Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China;National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;

关键词: Cyprinus carpio L.;    Wild population;    Domesticated strain;    COII Gene;    Genetic variation;   

DOI  :  

来源: DOAJ

【 摘 要 】

196 common carp (Cyprinus carpio L.) samples from five wild populations and ten domestic strains (six Chinese and four European) in China were collected and the genetic variation was analyzed using direct DNA sequencing of mitochondrial Cytochrome c oxidase subunit 2 gene (COII). As a result, a total of 22 haplotypes were identified, and one maternal ancestral haplotype (Hap_1) distributed in all populations exclusive of Songpu carp (SPC) and Hainan wild carp (HWC). For the other two major haplotypes, Hap_3 was mainly shared by German mirror carp (GMC) and populations from Amur River Basin, whereas Hap_16 appeared mainly in scatted scaled mirror carp (SSMC), Xingguo red carp (XGR), Songpu carp (SPC), and part of Yangtze River wild carp (YRWC). Although the genetic variance among populations accounted for only 27.79 % of the total variance, significant differentiation between HWC and other populations were spotted. The apparent split between HWC and other Eurasian populations supported the hypothesis that C. carpio emerged in East Asia as a species. Chinese wild populations exhibited highest genetic diversity (Hd = 0.801 ± 0.032; π = 0.0031 ± 0.0003), followed by introduced European strains (Hd = 0.735 ± 0.036; π = 0.0020 ± 0.0002) and Chinese domestic varieties (Hd = 0.657 ± 0.039, π = 0.0016 ± 0.0003). Significant negative Tajima’s D value was only found in SPC, which was probably the consequence of small founder size and subsequent population expansion.

【 授权许可】

Unknown