microRNA Expression in Women With and Without Polycystic Ovarian Syndrome Matched for Body Mass Index

Frontiers in Endocrinology

Soha R. Dargham¹  Nigel J. Gooderham¹  Thozhukat Sathyapalan²  Stephen L. Atkin³  Rhiannon David⁴  Manasi Benurwar⁴  Alexandra E. Butler⁵  Shahina Hayat⁶  Vimal Ramachandran⁶  S. Hani Najafi-Shoushtari⁶ 

[1] Cancer, Faculty of Medicine, Imperial College, London, United Kingdom;Academic Diabetes, Endocrinology and Metabolism, Hull York Medical School, University of Hull, Hull, United Kingdom;Department of Cell and Developmental Biology, Weill Cornell Medicine, New York, NY, United States;;Department of Surgery &Diabetes Research Center (DRC), Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), Doha, Qatar;Division of Research, Weill Cornell Medical College-Qatar, Qatar Foundation, Education City, Doha, Qatar;

关键词: microRNA;    polycystic ovarian syndrome;    follicular phase;    menstrual cycle;    insulin resistance;    androgens;   

DOI  :  10.3389/fendo.2020.00206

来源: DOAJ

【 摘 要 】

Background: Despite several authors who have hypothesized that alterations of small noncoding RNAs (miR) are implicated in the etiopathogenesis of polycystic ovarian syndrome (PCOS), contrasting findings have been reported so far. Discrepancies in body mass index (BMI) levels may account for these differences; therefore, the aim of the present study was to determine whether miR differed in serum samples collected from age- and BMI-matched control and PCOS women.Methods: In a cross-sectional study, miR were measured using quantitative polymerase chain reaction in 29 women with anovulatory PCOS women and 29 control women who were in the follicular phase of their menstrual cycle, from the local biobank.Results: One hundred seventy-six miR were detected, of which 15 miR passed the false discovery rate (FDR; p < 0.05) that differed between PCOS and control women. There was no association of the top 9 miR (p < 0.02) (miR-486-5p, miR-24-3p, miR-19b-3p, miR-22-3p, miR-19a-3p, miR-339-5p, miR-185-5p, miR-101-3p, miR-let-7i-5p) with BMI, androgen levels, insulin resistance, or antimullerian hormone (AMH) in either PCOS or normal women. Ingenuity pathway assessment showed the pathways were interrelated for abnormalities of the reproductive system.Conclusion: When the confounding influence of weight was accounted for, miR levels differed between anovulatory PCOS women and control women in the follicular phase of the menstrual cycle. Interestingly, the differing miR were associated with the pathways of reproductive abnormalities but did not associate with AMH or metabolic parameters.

【 授权许可】

Unknown