High-intensity interval training remodels the proteome and acetylome of human skeletal muscle

eLife

Ben Stocks¹  Alba Gonzalez-Franquesa¹  Julia Prats Quesada¹  Jeppe Kjærgaard Larsen¹  Atul Shahaji Deshmukh¹  Jens Bangsbo²  Martin Thomassen²  Anders Krogh Lemminger²  Morten Hostrup²  Brian Tate Weinert³ 

[1] Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark;The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Copenhagen, Denmark;

关键词: exercise;    skeletal muscle;    acetylation;    proteomics;    mitochondria;    calcium sensitivity;   

DOI  :  10.7554/eLife.69802

来源: DOAJ

【 摘 要 】

Exercise is an effective strategy in the prevention and treatment of metabolic diseases. Alterations in the skeletal muscle proteome, including post-translational modifications, regulate its metabolic adaptations to exercise. Here, we examined the effect of high-intensity interval training (HIIT) on the proteome and acetylome of human skeletal muscle, revealing the response of 3168 proteins and 1263 lysine acetyl-sites on 464 acetylated proteins. We identified global protein adaptations to exercise training involved in metabolism, excitation-contraction coupling, and myofibrillar calcium sensitivity. Furthermore, HIIT increased the acetylation of mitochondrial proteins, particularly those of complex V. We also highlight the regulation of exercise-responsive histone acetyl-sites. These data demonstrate the plasticity of the skeletal muscle proteome and acetylome, providing insight into the regulation of contractile, metabolic and transcriptional processes within skeletal muscle. Herein, we provide a substantial hypothesis-generating resource to stimulate further mechanistic research investigating how exercise improves metabolic health.

【 授权许可】

Unknown