Preparation of Nanoemulsions of Vitamin A and C by Microfluidization: Efficacy on the Expression Pattern of Milk-Specific Proteins in MAC-T Cells

Molecules

Tai-Young Hur¹  Kwang-Seok Ki¹  Mayakrishnan Vijayakumar¹  Dong-Hyun Lim¹  Sang-Bum Kim¹  Seong-Min Park¹  Tae-Il Kim¹  Tae-Gyun Kim²  Young-Jun Kim²  Eung-Gi Kwon³ 

[1] Dairy Science Division, National Institute of Animal Science, Rural Development Administration, Chungcheongnam-do, Cheonan 31000, Korea;Department of Food and Biotechnology, Korea University, 2511 Sejong-ro, Sejong 30019, Korea;Hanwoo Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang-gun, Gangwon-do 25340, Korea;

关键词: nanoemulsion;    microfluidization;    vitamins;    casein;   

DOI  :  10.3390/molecules24142566

来源: DOAJ

【 摘 要 】

In this study, we prepared stabilized vitamin A and C nanoemulsions, and investigated their efficacy on milk-specific proteins in bovine mammary epithelial cells (MAC-T). Emulsions of vitamin A (vit-A) and C (vit-C) were prepared using Lipoid S 75 and microfluidization. The particle size and polydispersity index (PDI) of nanoemulsified vit-A and vit-C were studied. The cytotoxic effect of nanoemulsion-free and nanoemulsified vit-A and vit-C was determined by an MTT assay. In addition, the efficacy of nanoemulsified vit-A and vit-C on the in vitro expression pattern of milk-specific proteins in MAC-T cells was investigated by quantitative RT-PCR. The results showed that the efficacies of stabilized nanoemulsions of vit-A and vit-C were 100% and 92.7%, respectively. The particle sizes were around 475.7 and 225.4 nm, and the zeta potentials were around −33.5 and −21.3 mV, respectively. The expression changes of α_s2-, β- and κ-casein were higher in the presence of a stabilized nanoemulsion of vit-A, compared with nanoemulsion-free vit-A. Furthermore, the expression changes of α_s2- and β-casein were lower and that of κ-casein was higher in the presence of a stabilized nanoemulsion of vit-C, compared with nanoemulsion-free vit-C. Thus, our findings demonstrate the efficacy of nanoemulsified vit-A and vit-C in changing the expression of milk-specific proteins in MAC-T cells.

【 授权许可】

Unknown