Characterization of fertility alteration and marker validation for male sterility genes in novel PTGMS lines hybrid rice

Saudi Journal of Biological Sciences

Mahmoud A. El-Hity¹  Rizk M. Abdallah²  Amr M. Reda²  E.S. El-Degwy³  Muneera D.F. AlKahtani³  Kotb A. Attia⁴  Latifa Al Husnain⁴  Hamdi F. El-Mowafi⁴  A.A. Hadifa⁴  Hussah I.M. AlGwaiz⁴ 

[1] Corresponding authors at: Center of Excellence in Biotechnology Research, King Saud University, Riyadh POX 2455-11451, Saudi Arabia.;Agronomy Department, College of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33512, Egypt;Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 102275, Riyadh 11675, Saudi Arabia;Rice Department, Field Crops Research Institute, ARC, Sakha, Kafr El-Sheikh 33717, Egypt;

关键词: Fertility alteration;    Hybrid rice;    PTGMS;    Sterility genes;    Molecular markers;   

DOI  :  

来源: DOAJ

【 摘 要 】

Photoperiod and thermosensitive genetic male sterile (PTGMS) lines have become one of the main sources of global rice production increasing. This study was conducted to evaluate the fertility alteration and validate the male sterility genes using validation markers in novel Egyptian Indica and Japonica PTGMS lines under natural conditions. The study revealed that the new genetic male sterile lines belong to the type of photo–thermosensitive genetic male sterility (PTGMS). The fertility alteration of these lines has influenced by photoperiod and temperature interaction. The new PTGMS lines have three sensitive periods of fertility alteration; transformation, sterility, and fertility period. Furthermore, the sensitive stage of fertility transformation might be from secondary branch primordial to pollen mother cells (PMC) meiosis. Under the natural Sakha condition, the new PTGMS lines were stable sterile under the condition of day length upper 13,75 h and temperature over 25 °C, while its convert to fertile under day length under 13 h, and temperature lower than 24 °C. The co-dominant markers identified the pms3 and tms5 genes in the new PTGMS lines, indicated that the fertility alteration in these lines controlled by photoperiod and thermosensitive stages.

【 授权许可】

Unknown