The phenotypic predisposition of the parent in F1 hybrid is correlated with transcriptome preference of the positive general combining ability parent

BMC Genomics

Daichang Yang¹  Yingguo Zhu¹  Wei Wang¹  Daiming Jiang¹  Xuefeng Qu¹  Zhenwei Liu¹  Zhibin Guo¹  Gaoyuan Song¹ 

[1] State Key Laboratory of Hybrid Rice and College of Life Sciences, Wuhan University, Luojia Hill, Wuhan 430072, Hubei Province, China

关键词: Diallelic crosses;    Transcriptome and phenotype bias;    Hybrid rice;    Transcriptome profiling;    General combining ability (GCA);   

Others  :  1217448
DOI  :  10.1186/1471-2164-15-297

 received in 2014-01-20, accepted in 2014-04-10,  发布年份 2014

【 摘 要 】

Background

Sprague and Tatum (1942) introduced the concepts of general combining ability (GCA) and specific combining ability (SCA) to evaluate the breeding parents and F1 hybrid performance, respectively. Since then, the GCA was widely used in cross breeding for elite parent selection. However, the molecular basis of GCA remains to unknown.

Results

We studied the transcriptomes of three varieties and three F1 hybrids using RNA-Sequencing. Transcriptome sequence analysis revealed that the transcriptome profiles of the F1s were similar to the positive GCA-effect parent. Moreover, the expression levels of most differentially expressed genes (DEGs) were equal to the parent with a positive GCA effect. Analysis of the gene expression patterns of gibberellic acid (GA) and flowering time pathways that determine plant height and flowering time in rice validated the preferential transcriptome expression of the parents with positive GCA effect. Furthermore, H3K36me3 modification bias in the Pseudo-Response Regulators (PRR) gene family was observed in the positive GCA effect parents and demonstrated that the phenotype and transcriptome bias in the positive GCA effect parents have been epigenetically regulated by either global modification or specific signaling pathways in rice.

Conclusions

The results revealed that the transcriptome profiles and DEGs in the F1s were highly related to phenotype bias to the positive GCA-effect parent. The transcriptome bias toward high GCA parents in F1 hybrids attributed to H3K36me3 modification both on global modification level and specific signaling pathways. Our results indicated the transcriptome profile and epigenetic modification level bias to high GCA parents could be the molecular basis of GCA.

【 授权许可】

   
2014 Song et al.; licensee BioMed Central Ltd.

【 图 表 】

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