Marker-assisted breeding of onion (A. cepa L.) maintainer line resistant to Downy mildew

Ovoŝi Rossii

G. F. Monakhos¹  Ya. T. Eidlin²  S. G. Monakhos² 

[1] Limited company «Breeding station after N. N. Timofeev»;Russian State Agrarian University – Moscow Timiryazev Agricultural Academy (RSAU-MTAA);

关键词: resistance gene;    maintainer line;    onion;    line;    marker-assisted selection;    molecular marker;    male sterility;    downy mildew;    cytoplasm;    peronospora destructor;   

DOI  :  10.18619/2072-9146-2021-3-34-39

来源: DOAJ

【 摘 要 】

Relevance. F1 hybrids breeding programs of onion are based on the use of nuclear cytoplasmic male sterility (NCMS). Breeding and seed production based on NCMS suggest a development of a female sterile maternal line, a maintainer line and a paternal component. The development of an isogenic pair of sterile line – maintainer line is one of the most labor-, time-consuming and intellectually-intensive stages of a breeding program, the implementation of which becomes more complicated with an increase in the number of traits / genes for which selection is carried out.Methods and results. This paper presents a genetic scheme for the development of a Downy mildewresistant (pathogen P. destructor) maintainer line of onion using marker-assisted selection (MAS). F2 populations were obtained from the cross of the female maintainer inbred line, Bn1- (13) (genotype – cytN msms pdpd) and male inbred line resistant to Downy mildew, No. 136 (genotype – cytT MsMs PdPd). DNA marker DMR1 were used for Downy mildew resistance gene Pd, jnurf13 – for maintainer gene ms and marker system 5`cob: orfA501 – for N, S and T cytoplasms. It was shown that the MAS application allow to develop a stable Downy mildew resistant maintainer line cytN msms PdPd in two generations.

【 授权许可】

Unknown