Populus tomentiglandulosa Extract Is Rich in Polyphenols and Protects Neurons, Astrocytes, and the Blood-Brain Barrier in Gerbil Striatum Following Ischemia-Reperfusion Injury

Molecules

Joon-Ha Park¹  Dae-Won Kim²  Soo-Young Choi³  Tae-Kyeong Lee³  Hyung-Il Kim⁴  Jun-Hwi Cho⁵  II-Jun Kang⁶  Ji-Hyeon Ahn⁷  Jae-Chul Lee⁷  Moo-Ho Won⁷  Jong-Dai Kim⁸ 

[1]Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju 38066, North Gyeongsang, Korea

[2]Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung–Wonju National University, Gangneung 25457, Gangwon, Korea

[3]Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Gangwon, Korea

[4]Department of Emergency Medicine, Dankook University Hospital, College of Medicine, Dankook University, Cheonan 31116, Chungnam, Korea

[5]Department of Emergency Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon 24289, Gangwon, Korea

[6]Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Gangwon, Korea

[7]Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 24341, Gangwon, Korea

[8]Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon 24341, Gangwon, Korea

关键词: astrocyte endfeet;    BBB leakage;    gliosis;    immunoglobulin G;    ischemia-reperfusion injury;    neuronal damage/death;   

DOI  :  10.3390/molecules26185430

来源: DOAJ

【 摘 要 】

Transient ischemia in brains causes neuronal damage, gliosis, and blood–brain barrier (BBB) breakdown, which is related to ischemia-induced brain dysfunction. Populus species have various pharmacological properties including antioxidant and anti-inflammatory activities. In this study, we found that phenolic compounds were rich in Populus tomentiglandulosa extract and examined the effects of Populus tomentiglandulosa extract on neuronal damage/death, astrogliosis, and BBB breakdown in the striatum, which is related to motor behavior, following 15-min transient ischemia in the forebrain in gerbils. The gerbils were pre-treated with 50, 100, and 200 mg/kg of the extract. The latter showed significant effects against ischemia-reperfusion injury. Ischemia-induced hyperactivity using spontaneous motor activity test was significantly attenuated by the treatment. Striatal cells (neurons) were dead at five days after the ischemia; however, pre-treatment with the extract protected the striatal cells from ischemia/reperfusion injury. Ischemia-induced reactive astrogliosis was significantly alleviated, in particular, astrocyte end feet, which are a component of BBB, were significantly preserved. Immunoglobulin G, which is not found in intact brain parenchyma, was apparently shown (an indicator of extravasation) in striatal parenchyma at five days after the ischemia, but IgG leakage was dramatically attenuated in the parenchyma by the pre-treatment. Based on these findings, we suggest that Populus tomentiglandulosa extract rich in phenolic compounds can be employed as a pharmaceutical composition to develop a preventive material against brain ischemic injury.

【 授权许可】

Unknown