Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers

Crystals

Suraj Pandey¹  Ishwor Poudyal¹  TekNarsingh Malla¹ 

[1] Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA;

关键词: time-resolved crystallography;    pump-probe serial femtosecond crystallography;    X-ray free electron lasers;    diffraction before destruction;    difference electron density map;    power titration;   

DOI  :  10.3390/cryst10070628

来源: DOAJ

【 摘 要 】

With time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond timescales. Here, we review the recent developments, opportunities, and challenges of pump-probe TRX at XFELs.

【 授权许可】

Unknown