Statistical optimization of harvesting Chlorella vulgaris using a novel bio-source, Strychnos potatorum

Biotechnology Reports

Sirajunnisa Abdul Razack¹  A.S. Santhalin Shellomith¹  Surendhiran Duraiarasan¹  Keerthana Muralikrishnan² 

[1] Bioprocess Laboratory, Department of Chemical Engineering, Annamalai University, Annamalainagar, Tamilnadu 608002, India;Department of Biotechnology, Bharathidasan Institute of Technology, Anna University, Tiruchirapalli, Tamilnadu 620024, India;

关键词: Chlorella vulgaris;    Bioflocculation;    Strychnos potatorum;    Response Surface Methodology;    Trypan Blue;   

DOI  :  10.1016/j.btre.2015.06.006

来源: DOAJ

【 摘 要 】

The present study was aimed at harvesting microalga, Chlorella vulgaris, by bioflocculation using seed powder of clearing nut, Strychnos potatorum. The research was essentially the prime step to yield a large biomass for utilising the cells in biodiesel production. Optimization of the parameters influencing bioflocculation was carried out statistically using RSM. The optimized conditions were 100 mg L−1 bioflocculant concentration, 35 °C temperature, 150 rpm agitation speed and 30 min incubation time and resulted in a maximum efficiency of 99.68%. Through cell viability test, using Trypan blue stain, it was found that cells were completely intact when treated with bioflocculant, but destroyed when exposed to chemical flocculant, alum. The overall study represented that S. potatorum could potentially be a bioflocculant of microalgal cells and a promising substitute for expensive and hazardous chemical flocculants. Moreover, this bioflocculant demonstrated their utility to harvest microalgal cells by economically, effectively and in an ecofriendly way.

【 授权许可】

Unknown