Phenotypic Characterization of Sulfolobus islandicus Strains Lacking the B-Family DNA Polymerases PolB2 and PolB3 Individually and in Combination

Frontiers in Microbiology

Stephen D. Bell¹  Peter B. Bohall¹ 

[1] Department of Biology, Indiana University, Bloomington, IN, United States;Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, United States;

关键词: Sulfolobus;    archaea;    DNA replication;    DNA damage;    polymerase;   

DOI  :  10.3389/fmicb.2021.666974

来源: DOAJ

【 摘 要 】

Across the three domains of life, B-family DNA polymerases play a variety of roles in both DNA repair and DNA replication processes. We examine the phenotypic consequences of loss of the putative repair polymerases PolB2 and/or PolB3 in the crenarchaeon Sulfolobus islandicus. We detect a modest growth advantage when cells lacking the polymerase are grown in unperturbed conditions. Further, we observe a striking insensitivity of the mutant lines to acute treatment with the oxidizing agent, hydrogen peroxide. In addition, cells lacking PolB3 show enhanced sensitivity to the DNA damaging agent 4-NQO. Our data therefore suggest that these non-essential DNA polymerases may influence DNA repair pathway choice in these hyperthermophilic aerobes.

【 授权许可】

Unknown