Genotyping of Mycobacterium leprae for understanding the distribution and transmission of leprosy in endemic provinces of China

International Journal of Infectious Diseases

Liangbin Yan¹  Haiqin Jiang¹  Limei Shen¹  Yanfei Kuang¹  Jinlan Li¹  Meiwen Yua²  Tiejun Shui²  Huan Chen²  Jun He²  Lemuel Shui Lun Tsang²  Yanqing Chen³  Balaji Pathakumari³  Le Wang³  Santosh Chokkakula⁴  Hao Wang⁴  Bin Li⁴  Wenyue Zhang⁴  Xiong Li⁴  Varalakshmi Vissa⁴  Hongsheng Wang⁴  Ying Shi⁵  Jie Liu⁶  De Wang⁶  Jun Yang⁶  Naveen Chandra Suryadevara⁶ 

[1] Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China;National Centre for STD and Leprosy Control, China CDC, Nanjing, China;Guizhou Provincial CDC, Guiyang, China;Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China;Vanderbilt University Medical Centre, Nashville, TN, USA;Yunnan Provincial CDC, Kunming, China;

关键词: Leprosy;    Mycobacterium leprae;    Molecular epidemiology;    VNTR;    SNP;    Strain typing and transmission;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: Understanding the nature of Mycobacterium leprae transmission is vital to implement better control strategies for leprosy elimination. The present study expands the knowledge of county-level strain diversity, distribution, and transmission patterns of leprosy in endemic provinces of China. Methods: We genetically characterized 290 clinical isolates of M. leprae from four endemic provinces using variable number tandem repeats (VNTR) and single nucleotide polymorphisms (SNPs). Attained genetic profiles and cluster consequences were contrasted with geographical and migration features of leprosy at county levels. Results: Considering the allelic variability of 17 VNTR loci by the discriminatory index, (GTA)9, (AT)17, (AT)15, (TA)18, (TTC)21, and (TA)10 are reported to be more highly polymorphic than other loci. The VNTR profile generated the low-density clustering pattern in the counties of Sichuan and Yunnan, whereas clusters have been observed from the isolates from Huayuan (N = 6), Yongding (N = 3), Zixing (N = 3), Chenxi (N = 2) and Zhongfang (N = 2) counties of Hunan, and Zhijin (N = 3), Anlong (N = 2), Zhenning (N = 2), and Xixiu (N = 2) counties of Guizhou. In some clusters, people’s social relations have been observed between villages. From the 290 clinical isolates, the most predominantly reported SNP was 3K (278, 95.8%), followed by SNP 1D (10, 3.4%), which are typically observed to be predominant in China. We also detected the novel SNP 3J (2, 0.8%), which has not yet been reported in China. Conclusion: The clustering pattern of M. leprae indicates the transmission of leprosy still persists at county levels, suggesting that there is a need to implement better approaches for tracing the close contacts of leprosy patients.

【 授权许可】

Unknown