Genome sequence and Carbohydrate Active Enzymes (CAZymes) repertoire of the thermophilic Caldicoprobacter algeriensis TH7C1T

Microbial Cell Factories

Jorge Barriuso¹  José Luis García¹  Amel Bouanane Derenfed²  Sawssan Neifar³  Samir Bejar³  Rihab Ameri³  Sonia Mhiri³  Nadia Zaraî Jaouadi³  Monia Mezghanni³  Nathalie Pradel⁴ 

[1] Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas (CIB-CSIC);Laboratoire de Biologie Cellulaire et Moléculaire (Équipe de Microbiologie), Université des Sciences et de la Technologie Houari Boumédiènne;Laboratory of Microbial Biotechnology, Enzymatic and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax;Université de Toulon, CNRS, IRD, MIO, Aix Marseille Univ;

关键词: Caldicoprobacter algeriensis TH7C1T;    CAZymes, CGC;    Biotechnological applications;   

DOI  :  10.1186/s12934-022-01818-0

来源: DOAJ

【 摘 要 】

Abstract Background Omics approaches are widely applied in the field of biology for the discovery of potential CAZymes including whole genome sequencing. The aim of this study was to identify protein encoding genes including CAZymes in order to understand glycans-degrading machinery in the thermophilic Caldicoprobacter algeriensis TH7C1T strain. Results Caldicoprobacter algeriensis TH7C1T is a thermophilic anaerobic bacterium belonging to the Firmicutes phylum, which grows between the temperatures of 55 °C and 75 °C. Next generation sequencing using Illumina technology was performed on the C. algeriensis strain resulting in 45 contigs with an average GC content of 44.9% and a total length of 2,535,023 bp. Genome annotation reveals 2425 protein-coding genes with 97 ORFs coding CAZymes. Many glycoside hydrolases, carbohydrate esterases and glycosyltransferases genes were found linked to genes encoding oligosaccharide transporters and transcriptional regulators; suggesting that CAZyme encoding genes are organized in clusters involved in polysaccharides degradation and transport. In depth analysis of CAZomes content in C. algeriensis genome unveiled 33 CAZyme gene clusters uncovering new enzyme combinations targeting specific substrates. Conclusions This study is the first targeting CAZymes repertoire of C. algeriensis, it provides insight to the high potential of identified enzymes for plant biomass degradation and their biotechnological applications.

【 授权许可】

Unknown