AP-64, Encoded by C5orf46, Exhibits Antimicrobial Activity against Gram-Negative Bacteria

Biomolecules

Yuelong Wang¹  Liangxue Zhou¹  Jianhan Huang¹  Hui Yang²  Xi Zheng³  Kunhong Zhong⁴  Aiping Tong⁴  Wenhao Guo⁴  Gang Guo⁴  Hexian Li⁴  Shasha Zhao⁴  Zongliang Zhang⁴  Zeng Wang⁴ 

[1] Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China;Department of Otolaryngology, Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, China;Lung Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China;State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China;

关键词: antimicrobial peptides;    AMPs;    Gram-negative bacteria;    C5orf46;    AP-64;   

DOI  :  10.3390/biom11040485

来源: DOAJ

【 摘 要 】

Antimicrobial peptides (AMPs), which are evolutionarily conserved components of the innate immune response, contribute to the first line of defense against microbes in the skin and at mucosal surfaces. Here, we report the identification of a human peptide, encoded by the chromosome 5 open reading frame 46 (C5orf46) gene, as a type of AMP, which we termed antimicrobial peptide with 64 amino acid residues (AP-64). AP-64 is an anionic amphiphilic peptide lacking cysteines (MW = 7.2, PI = 4.54). AP-64 exhibited significant antibacterial activity against Gram-negative bacteria, including Escherichia coli DH5α, Escherichia coli O157:H7, Vibrio cholerae, and Pseudomonas aeruginosa. Moreover, AP-64 was efficient in combating Escherichia coli O157:H7 infections in a mouse model and exhibited cytotoxic effects against human T-cell lymphoma Jurkat and B-cell lymphoma Raji cells. We also observed that Gm94, encoded by mouse C5orf46 homologous gene, closely resembles AP-64 in its antibacterial properties. Compared with other human AMPs, AP-64 has distinct characteristics, including a longer sequence length, absence of cysteine residues, a highly anionic character, and cell toxicity. Together, this study identified that AP-64 is an AMP worthy of further investigation.

【 授权许可】

Unknown